Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Hamster (at least 90% immunogen sequence identity)
Protein A purified
IHC - Paraffin (10 µg/ml)
Western blot (1:1000)
Specificity and Use
PDIA4 / ERP72 antibody was raised against a 16 residue synthetic peptide (C)FIDEHATKRSRTKEEL based on the mouse ERp72 (residues 623-638) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster (94%); Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%); Pig, Platypus (81%).
Detects an ~72 kD protein, corresponding to the apparent molecular mass of ERp72.
Immunohistochemistry: LS-B3756 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3756 was determined to be 10 ug/ml.