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Anti-PDI / P4HB Antibody IHC-plus™ LS-B2372

Note: This antibody replaces LS-C38114

Ordering

Wt. Vol. Conc. Price
- 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PDI / P4HB Antibodies

Anti-PDI / P4HB Antibody (clone 3-2B12) IHC-plus™ LS-B2162
Mouse Monoclonal [clone 3-2B12] (IgG1,k) to Human PDI / P4HB
Human
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PDI / P4HB Antibody IHC-plus™ LS-B2372
Rabbit Polyclonal (IgG) to Human PDI / P4HB
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PDI / P4HB Antibody (aa499-509) IHC-plus™ LS-B3137
Mouse Monoclonal (IgG1) to Rat PDI / P4HB
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PDI / P4HB
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human PDI / P4HB
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • ICC (1:50)
  • Immunofluorescence (1:50)
  • Western blot (1:1000)

Specificity and Use

PDI / P4HB antibody was raised against synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).
Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.
Immunohistochemistry: LS-B2372 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2372 was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.

Packaging

Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PDI / P4HB

P07237 NM_000918 NP_000909.2

P4HB Antibody, ERBA2L Antibody, DSI Antibody, GIT Antibody, p4HB Antibody, p4Hbeta Antibody, PDIA1 Antibody, PHDB Antibody, PO4DB Antibody, PROHB Antibody, Protocollagen hydroxylase Antibody, Protein disulfide-isomerase Antibody, PO4HB Antibody, PDI Antibody

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins.

Immunohistochemistry

Immunohistochemistry
Anti-P4HB antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2372 dilution 1:50.
 

Requested From: United States
Date Requested: 12/5/2016

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