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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDI / P4HB Antibody (clone 3‑2B12) IHC‑plus™ LS‑B2162 Best Seller
Note: This antibody replaces LS-C38385
P4HB antibody LS-B2162 is an unconjugated mouse monoclonal antibody to human P4HB (PDI). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Catalog
Size
Price
LS-B2162-50
50 µg (2 mg/ml)
Unavailable

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Product Description

P4HB antibody LS-B2162 is an unconjugated mouse monoclonal antibody to human P4HB (PDI). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
About PDI / P4HB
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. P07237 NM_000918 NP_000909.2

P4HB Antibody, ERBA2L Antibody, DSI Antibody, GIT Antibody, p4HB Antibody, p4Hbeta Antibody, PDIA1 Antibody, PHDB Antibody, PO4DB Antibody, PROHB Antibody, Protocollagen hydroxylase Antibody, Protein disulfide-isomerase Antibody, PO4HB Antibody, PDI Antibody


Specifications

Target
Human PDI / P4HB
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [3-2B12]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • IHC - Frozen (20 µg/ml)
  • Western blot (2.5 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PDI / P4HB antibody was raised against human prolyl 4-hydroxylase (hPH).
Specificity
Recognizes the b-subunit of human Prolyl Hydroxylase, 4.
Usage
Immunohistochemistry: LS-B2162 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2162 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.0, 2% BSA (protease free).
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (2)

1
Molecular markers of endometrial carcinoma detected in uterine aspirates. Colas E, Perez C, Cabrera S, Pedrola N, Monge M, Castellvi J, Eyzaguirre F, Gregorio J, Ruiz A, Llaurado M, Rigau M, Garcia M, Ertekin T, Montes M, Lopez-Lopez R, Carreras R, Xercavins J, Ortega A, Maes T, Rosell E, Doll A, Abal M, Reventos J, Gil-Moreno A. International journal of cancer. Journal international du cancer. 2011 Nov;129:2435-44. [Full Text Article] [PubMed:21207424]
2
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Ohe R, Aung NY, Meng H, Kabasawa T, Suto A, Tamazawa N, Yang S, Kato T, Yamakawa M. Leukemia & lymphoma. 2015 57:1687-96. (IHC; Human) [Full Text Article] [PubMed:26700650] [PMC:PMC4926777]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.

Immunohistochemistry

Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.

Immunohistochemistry

Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.

Immunohistochemistry

Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2162 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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