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Anti-PDI / P4HB Antibody (clone 3-2B12) IHC-plus™ LS-B2162
Note: This antibody replaces LS-C38385Most Popular PDI / P4HB Antibodies
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Details
| Human PDI / P4HB | |
| Mouse | |
| Human (tested or 100% immunogen sequence identity) | |
| IgG1,k Monoclonal [3-2B12] | |
| Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE. | |
| Purified | |
| Unmodified |
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- IHC - Frozen (20 µg/ml)
- Western blot (2.5 µg/ml)
- ELISA
- (applications tested for the base form of this product only)
Specificity and Use
| PDI / P4HB antibody was raised against human prolyl 4-hydroxylase (hPH). | |
| Recognizes the b-subunit of human Prolyl Hydroxylase, 4. | |
| Immunohistochemistry: LS-B2162 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2162 was determined to be 2.5 ug/ml. |
Packaging
| PBS, pH 7.0, 2% BSA (protease free). | |
| Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles. | |
| For research use only. |
Publications (2)
1
Molecular markers of endometrial carcinoma detected in uterine aspirates. Colas E, Perez C, Cabrera S, Pedrola N, Monge M, Castellvi J, Eyzaguirre F, Gregorio J, Ruiz A, Llaurado M, Rigau M, Garcia M, Ertekin T, Montes M, Lopez-Lopez R, Carreras R, Xercavins J, Ortega A, Maes T, Rosell E, Doll A, Abal M, Reventos J, Gil-Moreno A. International journal of cancer. Journal international du cancer. 2011 Nov;129:2435-44. [Full Text Article]
[PubMed:21207424]
2
Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Ohe R, Aung NY, Meng H, Kabasawa T, Suto A, Tamazawa N, Yang S, Kato T, Yamakawa M. Leukemia & lymphoma. 2015 57:1687-96. (IHC; Human)
[Full Text Article]
[PubMed:26700650]
[PMC:PMC4926777]
About PDI / P4HB
P07237
NM_000918
NP_000909.2
P4HB Antibody, ERBA2L Antibody, DSI Antibody, GIT Antibody, p4HB Antibody, p4Hbeta Antibody, PDIA1 Antibody, PHDB Antibody, PO4DB Antibody, PROHB Antibody, Protocollagen hydroxylase Antibody, Protein disulfide-isomerase Antibody, PO4HB Antibody, PDI Antibody
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins.
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Requested From:
Date Requested: 4/26/2018
Date Requested: 4/26/2018
