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Anti-PDI / P4HB Antibody (aa499-509) IHC-plus™ LS-B3137

Note: This antibody replaces LS-C38113
Catalog Size Price
LS-B3137-50 50 µg (1 mg/ml) Unavailable

Related Products

74 PDI / P4HB Antibodies

Most Popular PDI / P4HB Antibodies

Anti-PDI / P4HB Antibody (clone 3-2B12) IHC-plus™ LS-B2162
Mouse Monoclonal [clone 3-2B12] (IgG1,k) to Human PDI / P4HB
Human
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PDI / P4HB Antibody IHC-plus™ LS-B2372
Rabbit Polyclonal (IgG) to Human PDI / P4HB
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PDI / P4HB Antibody (aa499-509) IHC-plus™ LS-B3137
Mouse Monoclonal (IgG1) to Rat PDI / P4HB
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PDI / P4HB Antibody (FITC) LS-C183228
Rabbit Polyclonal (IgG) to Human PDI / P4HB
Human
Western blot, ELISA
FITC Conjugated
Anti-PDI / P4HB Antibody (clone 1D3, PE) LS-C343618
Mouse Monoclonal [clone 1D3] (IgG1) to Rat PDI / P4HB
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Rabbit, Sheep, Chicken, Xenopus, Porcine
Flow Cytometry
PE Conjugated

100% Guaranteed
Mouse Monoclonal (IgG1) to Rat PDI / P4HB
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Rat PDI / P4HB
Mouse
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot

Specificity and Use

PDI / P4HB antibody was raised against synthetic peptide corresponding to aa499-509 of rat protein disulfide isomerase (KLH coupled).
aa499-509
Recognizes rat protein disulfide isomerase at ~58kD.
Immunohistochemistry: LS-B3137 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3137 was determined to be 5 ug/ml. Positive control: HeLa Heat Shocked Cell Lysate, MCF-7 cell lysate.

Packaging

PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About PDI / P4HB

P07237 NM_000918 NP_000909.2

P4HB Antibody, ERBA2L Antibody, DSI Antibody, GIT Antibody, p4HB Antibody, p4Hbeta Antibody, PDIA1 Antibody, PHDB Antibody, PO4DB Antibody, PROHB Antibody, Protocollagen hydroxylase Antibody, Protein disulfide-isomerase Antibody, PO4HB Antibody, PDI Antibody

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins.

Immunohistochemistry

Immunohistochemistry
Anti-P4HB antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3137 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3137 concentration 5 ug/ml.

Requested From: 
Date Requested: 6/26/2017

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