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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDHA1 / PDH E1 Alpha Antibody LS‑C163477
PDH E1 Alpha antibody LS-C163477 is an unconjugated rabbit polyclonal antibody to human PDH E1 Alpha (PDHA1). Validated for WB.
Catalog
Size
Price
LS-C163477-400
400 µl
Unavailable

Popular PDHA1 / PDH E1 Alpha Products

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Rabbit
Applications: Western blot
Pyruvate Dehydrogenase E1-alpha subunit [phospho Ser293] Antibody - Detection of PDHE1 (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33248.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Pyruvate Dehydrogenase E1-alpha subunit [phospho Ser293] Antibody - Detection of PDHE1 (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33248.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Anti-PDHA1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: ELISA, Dot Blot

Product Description

PDH E1 Alpha antibody LS-C163477 is an unconjugated rabbit polyclonal antibody to human PDH E1 Alpha (PDHA1). Validated for WB.
About PDHA1 / PDH E1 Alpha
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). P08559 NM_000284 NP_000275.1

PDHA1 Antibody, PDHCE1A Antibody, PDHA Antibody, PHE1A Antibody, PDHE1-A type I Antibody


Specifications

Target
Human PDHA1 / PDH E1 Alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.
Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.

Western blot

PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).
PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).

Western blot

Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.
Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.

Western blot

PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).
PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).

Western blot

Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.
Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.

Western blot

PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).
PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).

Western blot

Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.
Western blot of PDHA1 (arrow) using rabbit polyclonal PDHA1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene.

Western blot

PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).
PDHA1 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The PDHA1 antibody detected the PDHA1 protein (arrow).


Requested From: 
Date Requested: 6/24/2018

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