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Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, DY550) LS-C141922

Ordering

Wt. Vol. Conc. Price
- 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PDHA1 / PDH E1 Alpha Antibodies

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293) LS-C141917
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, Biotin) LS-C141918
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human
ICC, Immunofluorescence, Western blot
Biotin Conjugated
Immunocytochemistry Image
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser232, PE) LS-C265964
Rabbit Polyclonal (IgG) to Human PDHA1 / PDH E1 Alpha
Human
Western blot, ELISA, Dot Blot
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human
ICC, Immunofluorescence, Western blot (applications tested for the base form of this product only)
DY550 Conjugated

Details

Human PDHA1 / PDH E1 Alpha
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
DyLight 550. Also available Unconjugated or conjugated with Biotin, DY650, DY488.
Immunoaffinity purified
Unmodified

Applications

  • ICC
  • Immunofluorescence
  • Western blot (1:2500 - 1:5000)
  • (applications tested for the base form of this product only)

Specificity and Use

PDHA1 / PDH E1 Alpha antibody was raised against a synthetic peptide surrounding the phosphorylated serine 293 of the human PDHE1 alpha protein. [Swiss-Prot #P08559]
pSer293
The phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and dilution.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium borate
Store at 4°C. Do not freeze.
For research use only.

About PDHA1 / PDH E1 Alpha

P08559 NM_000284 NP_000275.1

PDHA1 Antibody, PDHCE1A Antibody, PDHA Antibody, PHE1A Antibody, PDHE1-A type I Antibody

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Immunocytochemistry

Immunocytochemistry
Pyruvate Dehydrogenase E1-alpha subunit [phospho Ser293] Antibody - Detection of PDHE1 (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33248. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Pyruvate Dehydrogenase E1 alpha Phosphospecific Antibody - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/4/2016

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