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Anti-PDHA1 / PDH E1 Alpha Antibody (clone 2C10) LS-C172854

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.75 mg/ml $325
Inquire for larger quantities

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Most Popular PDHA1 / PDH E1 Alpha Antibodies

Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293) LS-C141917
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, Biotin) LS-C141918
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human
ICC, Immunofluorescence, Western blot
Biotin Conjugated
Immunocytochemistry Image
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser232, PE) LS-C265964
Rabbit Polyclonal (IgG) to Human PDHA1 / PDH E1 Alpha
Human
Western blot, ELISA, Dot Blot
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2C10] (IgG2b) to Human PDHA1 / PDH E1 Alpha
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human PDHA1 / PDH E1 Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [2C10]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

PDHA1 / PDH E1 Alpha antibody was raised against full length human recombinant protein of human PDHA1 (NP_000275) produced in HEK293T cell.
Human PDHA1

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About PDHA1 / PDH E1 Alpha

P08559 NM_000284 NP_000275.1

PDHA1 Antibody, PDHCE1A Antibody, PDHA Antibody, PHE1A Antibody, PDHE1-A type I Antibody

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Immunofluorescence

Immunofluorescence
Anti-PDHA1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.

Flow Cytometry

Flow Cytometry
Flow cytometry of Jurkat cells, using anti-PDHA1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-PDHA1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Requested From: United States
Date Requested: 12/7/2016

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