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Anti-PDHA1 / PDH E1 Alpha Antibody (clone 2C10) LS-C172854

Catalog Size Price
LS-C172854-100 100 µl (0.75 mg/ml) Unavailable

Most Popular PDHA1 / PDH E1 Alpha Antibodies

Anti-PDHA1 / PDH E1 Alpha Antibody (N-Terminus) LS-C81158
Rabbit Polyclonal (IgG) to Human PDHA1 / PDH E1 Alpha
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Rabbit
Western blot
Unconjugated
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293) LS-C141917
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot
Unconjugated
Pyruvate Dehydrogenase E1-alpha subunit [phospho Ser293] Antibody - Detection of PDHE1 (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33248.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser293, Biotin) LS-C141918
Rabbit Polyclonal to Human PDHA1 / PDH E1 Alpha
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot
Biotin Conjugated
Pyruvate Dehydrogenase E1-alpha subunit [phospho Ser293] Antibody - Detection of PDHE1 (Green) in HepG2 cells. Nuclei (Blue) are counterstained with Hoechst 33248.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDHA1 / PDH E1 Alpha Antibody (clone 2C10) LS-C172854
Mouse Monoclonal [clone 2C10] (IgG2b) to Human PDHA1 / PDH E1 Alpha
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Anti-PDHA1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1.
Anti-PDHA1 / PDH E1 Alpha Antibody (phospho-Ser232, PE) LS-C265964
Rabbit Polyclonal (IgG) to Human PDHA1 / PDH E1 Alpha
Human
ELISA, Dot Blot
PE Conjugated

100% Guaranteed
Mouse Monoclonal [clone 2C10] (IgG2b) to Human PDHA1 / PDH E1 Alpha
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human PDHA1 / PDH E1 Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [2C10]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

Full length human recombinant protein of human PDHA1 (NP_000275) produced in HEK293T cell.
Human PDHA1

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PDHA1 / PDH E1 Alpha

P08559 NM_000284 NP_000275.1

PDHA1 Antibody, PDHCE1A Antibody, PDHA Antibody, PHE1A Antibody, PDHE1-A type I Antibody

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Immunofluorescence

Anti-PDHA1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1.
Anti-PDHA1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-PDHA1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-PDHA1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-PDHA1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-PDHA1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Requested From: 
Date Requested: 5/24/2018

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