Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human PDGFRA / PDGFR Alpha
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot, Immunoprecipitation
Rat Monoclonal [clone APA5] (IgG2a,k) to Mouse PDGFRA / PDGFR Alpha
IHC - Frozen, Western blot, Flow Cytometry
Mouse PDGFRA / PDGFR Alpha
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [APA5]
IHC - Frozen
Specificity and Use
PDGFRA / PDGFR Alpha antibody was raised against mouse PDGFRA
The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stablizer
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development.
Staining of NIH/3T3 cells with 0.5 ug of Purified Rat IgG2a isotype control (open histogram) or 0.5 ug of Purified anti-mouse CD140a (APA5) (colored histogram) followed by Biotin Anti-Rat IgG and SAv-PE. Total viable cells were used for analysis.