Target
Human PDE4B
Synonyms
PDE4B | Pde-ivb | PDE32 | PDE4B5 | PDEIVB | Phosphodiesterase 4b | DPDE4 | PDE4B3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-IDIATEDKSPVDT, from the C-Terminus of protein sequence according to NP_002591.2NP_001032416.1NP_001032417.1.
Specificity
Human PDE4B. This antibody is expected to recognize all reported isoforms (NP_002591.2; NP_001032416.1; NP_001032417.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 98kD band observed in Human Uterus lysates (calculated MW of 83.3kD according to NP_002591.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mhats et al, Endocrinology. 1999 Jul;140(7):3228-37. PMID: 10385419). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee