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Anti-PDCD4 Antibody (phospho-Ser457, clone 9G6) LS-C154004


Wt. Vol. Conc. Price
100 µg - - Unavailable

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100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 9G6] (IgG) to Human PDCD4
Human, Mouse, Rat, Xenopus
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Human PDCD4
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
IgG Monoclonal [9G6]
Protein A purified


  • IHC - Paraffin (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)

Specificity and Use

PDCD4 antibody was raised against this monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Pdcd4 phospho S457 Antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kD in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009 284:18515-24. [PubMed:19419954] [PMC:PMC2709372]

About PDCD4

Q53EL6 NM_014456 NP_055271.2

PDCD4 Antibody, H731 Antibody, Nuclear antigen H731-like Antibody, Nuclear antigen H731 Antibody, Protein 197/15a Antibody

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. PDCD4 inhibits the translation initiation factor E1F4A.


Anti-Pdcd4 Antibody - Immunohistochemistry. Antibody LS-C154004 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti-Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.

Western blot

Western blot
PDCD4 antibody Western Blot. Western blot of Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1? Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4C in 1% BSA-TBS-T) followed by 2? Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M Lot# 20121 at 1:40000 in MB-070 30 min RT. Bands at ~62 kD and ~32 kD were detected.

Western blot

Western blot
Anti-Pdcd4 pS457 Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kD) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2000 dilution, overnight at 4C. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20000 dilution in blocking buffer (MB-070) for 1 h at 4C followed by visualization using a Biospectrum imaging system (UVP).

Requested From: 
Date Requested: 4/25/2017

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