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Anti-PDCD4 Antibody (phospho-Ser457, clone 9G6, Biotin) LS-C153791

Ordering

Wt. Vol. Conc. Price
50 µg 100 µl - $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PDCD4 Antibodies

Anti-PDCD4 Antibody (phospho-Ser457) IHC-plus™ LS-B487
Rabbit Polyclonal to Human PDCD4
Human, Mouse, Rat, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PDCD4 Antibody (aa1-469, clone K4C1) IHC-plus™ LS-B2949
Mouse Monoclonal [clone K4C1] (IgG1,k) to Human PDCD4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PDCD4 Antibody (aa210-221) IHC-plus™ LS-B9458
Goat Polyclonal to Human PDCD4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 9G6] (IgG) to Human PDCD4
Human, Mouse, Rat, Xenopus
Western blot, Immunoprecipitation, ELISA
Biotin Conjugated

Details

Human PDCD4
Mouse
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
IgG Monoclonal [9G6]
Biotin
Protein A purified
Unmodified

Applications

  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (1:1000 - 1:5000)
  • ELISA

Specificity and Use

PDCD4 antibody was raised against this monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
pSer457
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kD in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009 284:18515-24. [PubMed:19419954] [PMC:PMC2709372]

About PDCD4

Q53EL6 NM_014456 NP_055271.2

PDCD4 Antibody, H731 Antibody, Nuclear antigen H731-like Antibody, Nuclear antigen H731 Antibody, Protein 197/15a Antibody

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. PDCD4 inhibits the translation initiation factor E1F4A.

Western blot

Western blot
Western blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoforms This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

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