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Anti-PDCD1 / CD279 / PD-1 Antibody (clone J116) IHC-plus™ LS-B7883

Note: This antibody replaces LS-C106792

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PDCD1 / CD279 / PD-1 Antibodies

Anti-PDCD1 / CD279 / PD-1 Antibody (Internal) IHC-plus™ LS-B1387
Rabbit Polyclonal to Human PDCD1 / CD279 / PD-1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PDCD1 / CD279 / PD-1 Antibody (clone J116) IHC-plus™ LS-B7883
Mouse Monoclonal [clone J116] (IgG1,k) to Human PDCD1 / CD279 / PD-1
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone J116] (IgG1,k) to Human PDCD1 / CD279 / PD-1
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human PDCD1 / CD279 / PD-1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [J116]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

PDCD1 / CD279 / PD-1 antibody was raised against human PDCD1
The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
2-8 C.
For research use only.

About PDCD1 / CD279 / PD-1

Q15116 NM_005018 NP_005009.2

PDCD1 Antibody, CD279 Antibody, HPD-1 Antibody, HPD-l Antibody, PD1 Antibody, Protein PD-1 Antibody, SLEB2 Antibody, PD-1 Antibody, CD279 antigen Antibody, Programmed cell death 1 Antibody

PDCD1 / CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure.

Immunohistochemistry

Immunohistochemistry
Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7883 dilution 20 ug/ml.

Flow Cytometry

Flow Cytometry
Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry

Flow Cytometry
Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
 

Requested From: United States
Date Requested: 12/2/2016

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