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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDCD1 / CD279 / PD-1 Antibody (clone J116) IHC-plus™ LS-B7883

Best Seller
Note: This antibody replaces LS-C106792
PD-1 antibody LS-B7883 is an unconjugated mouse monoclonal antibody to human PD-1 (PDCD1 / CD279). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7883-50
50 µg (0.5 mg/ml)
$425
Description
PD-1 antibody LS-B7883 is an unconjugated mouse monoclonal antibody to human PD-1 (PDCD1 / CD279). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PDCD1 / CD279 / PD-1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
J116
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human PDCD1
Usage
The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About PDCD1 / CD279 / PD-1
Q15116 NM_005018 NP_005009.2

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Images

Immunohistochemistry

Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.

Flow Cytometry

Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry

Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Immunohistochemistry

Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.

Flow Cytometry

Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry

Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Immunohistochemistry

Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.

Flow Cytometry

Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry

Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Immunohistochemistry

Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.

Flow Cytometry

Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Flow Cytometry

Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Requested From: United States
Date Requested: 4/23/2019
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