Human PDCD1 / CD279 / PD-1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC4H6

Unconjugated. Also available conjugated with Biotin.

Protein A purified

Unmodified

Recombinant human CD279.

Anti-CD279 antibody clone ANC4H6 binds to CD279 present on PHA activated PBMC in FACS, and binds to recombinant CD279 in EIA. Pre incubation of recombinant CD279-coated plates with ANC4H6 blocks binding of biotinylated recombinant CD273 in EIA.

• Flow Cytometry

Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with 5 ug/ml Phytohemagglutanin (PHA). Five x 105 cells per tube were pre incubated 10 minutes with 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD279 antibody at 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.6 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Binding was blocked when antibody was pre incubated with a 10-fold excess of recombinant CD279-muIg.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15116 NCBI: NM_005018 NP_005009.2