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Catalog Number Size Price
LS-C357452-10 10 µg $318 
LS-C357452-100 100 µg (0.5 mg/ml) $470 
PDCD1 / CD279 / PD-1 Antibody - Western blot analysis of PD1/PDCD1 using anti-PD1/PDCD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW246. 7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/PDCD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PD1/PDCD1 at approximately 65KD. The expected band size for PD1/PDCD1 is at 32KD.
PDCD1 / CD279 / PD-1 Antibody - PD1 antibody , . All lanes: Anti PD1 at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Predicted band size: 32 kD . Observed band size: 38 kD.
PDCD1 / CD279 / PD-1 Antibody - PD1 antibody , . All lanes: Anti PD1 at 0.5 ug/ml. WB: Recombinant Human PD1 Protein 0.5ng . Predicted band size: 38 kD. Observed band size: 38 kD.
PDCD1 / CD279 / PD-1 Antibody - Western blot analysis of PD1/PDCD1 using anti-PD1/PDCD1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW246. 7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/PDCD1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PD1/PDCD1 at approximately 65KD. The expected band size for PD1/PDCD1 is at 32KD.
PDCD1 / CD279 / PD-1 Antibody - PD1 antibody , . All lanes: Anti PD1 at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Predicted band size: 32 kD . Observed band size: 38 kD.
PDCD1 / CD279 / PD-1 Antibody - PD1 antibody , . All lanes: Anti PD1 at 0.5 ug/ml. WB: Recombinant Human PD1 Protein 0.5ng . Predicted band size: 38 kD. Observed band size: 38 kD.
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Polyclonal Rabbit anti‑Human PDCD1 / CD279 / PD‑1 Antibody (aa101‑288, WB) LS‑C357452

Polyclonal Rabbit anti‑Human PDCD1 / CD279 / PD‑1 Antibody (aa101‑288, WB) LS‑C357452

Note: This antibody replaces LS-C389061
Antibody:
PDCD1 / CD279 / PD-1 Rabbit anti-Human Polyclonal (aa101-288) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357452-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDCD1 / CD279 / PD-1 Rabbit anti-Human Polyclonal (aa101-288) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PD-1 antibody LS-C357452 is an unconjugated rabbit polyclonal antibody to PD-1 (PDCD1 / CD279) (aa101-288) from human. It is reactive with human and rat. Validated for WB.
Target
Human PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human PD1 recombinant protein (Position: P101-L288). Human PD1 shares 59% amino acid (aa) sequence identity with mouse PD1.
Epitope
aa101-288
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD1 / CD279 / PD-1
Q15116 NM_005018 NP_005009.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024