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Antibodies > PCSK4 > LS-C313227
 
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Catalog Number Size Price
LS-C313227-10 10 µg $318 
LS-C313227-100 100 µg (0.5 mg/ml) $470 
PCSK4 Antibody - Western blot analysis of PCSK4 using anti-PCSK4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.
PCSK4 Antibody - WB of PCSK4 / PC4 antibody. WB: Rat Brain Tissue.
PCSK4 Antibody - Western blot analysis of PCSK4 using anti-PCSK4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.
PCSK4 Antibody - WB of PCSK4 / PC4 antibody. WB: Rat Brain Tissue.
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Polyclonal Rabbit anti‑Mouse PCSK4 Antibody (aa117‑133, WB) LS‑C313227

Polyclonal Rabbit anti‑Mouse PCSK4 Antibody (aa117‑133, WB) LS‑C313227

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Note: This antibody replaces LS-C389117
Antibody:
PCSK4 Rabbit anti-Mouse Polyclonal (aa117-133) Antibody
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313227-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PCSK4 Rabbit anti-Mouse Polyclonal (aa117-133) Antibody
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PCSK4 antibody LS-C313227 is an unconjugated rabbit polyclonal antibody to mouse PCSK4 (aa117-133). Validated for WB.
Target
Mouse PCSK4
Synonyms
PCSK4 | PC4 | Proprotein convertase 4 | SPC5
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of mouse Proprotein convertase PC4(117-133 aa DPWFSKQWYMNKEIQQD), different from the related rat sequence by one amino acid.
Epitope
aa117-133
Specificity
Expressed abundantly in the testis. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PCSK4
Q6UW60 BC036354 AAH36354.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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