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Anti-PCNA Antibody IHC-plus™ LS-B1931

Note: This antibody replaces LS-C33745
Catalog Size Price
LS-B1931-50 50 µg (0.2 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human PCNA
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human PCNA
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:200)

Specificity and Use

PCNA antibody was raised against recombinant human PCNA.
Recombinant human PCNA protein
Immunohistochemistry: LS-B1931 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1931 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
+4°C, avoid freezing.
For research use only.

About PCNA

P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1931 concentration 5 ug/ml.

Requested From: 
Date Requested: 12/15/2017

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