Rat PCNA

Mouse

Rat, Human, Monkey, Mouse, Pig, Chicken, Zebrafish, Drosophila, Yeast (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

SPM350

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Rat PCNA protein.

Rat PCNA

• IHC
• IHC - Paraffin (0.25 - 0.5 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P12004 NCBI: NM_002592 NP_002583.1