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Anti-PCNA Antibody (clone PC10) IHC-plus™ LS-B3434

Note: This antibody replaces LS-C49314

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PCNA Antibodies

Anti-PCNA Antibody (Internal) IHC-plus™ LS-B402
Rabbit Polyclonal to Human PCNA
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PCNA Antibody (C-Terminus) IHC-plus™ LS-B2826
Rabbit Polyclonal to Human PCNA
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-PCNA Antibody IHC-plus™ LS-B4230
Mouse Monoclonal (IgG2a) to Rat PCNA
Rat, Human, Insect, S. pombe, Vertebrate
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone PC10] to Rat PCNA
Rat, Human, Mouse, Chicken, Zebrafish, Drosophila, Yeast, Primate
IHC - Paraffin, Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Unconjugated

Details

Rat PCNA
Mouse
Rat, Human, Mouse, Chicken, Zebrafish, Drosophila, Yeast, Primate (tested or 100% immunogen sequence identity)
Monoclonal [PC10]
Unconjugated. Also available conjugated with DY650, DY488, DY550.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:5000)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)

Specificity and Use

PCNA antibody was raised against proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Immunohistochemistry: LS-B3434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3434 was determined to be 10 ug/ml.

Packaging

Tris-glycine, 150mM NaCl pH 7.5, 0.05% Sodium Azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PCNA

P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3434 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3434 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3434 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3434 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3434 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western blot of human (HeLa), murine (SV-T2), bovine (BAEC), and porcine (PAE) cell extracts using PCNA (PC10) Mouse mAb. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/6/2016

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