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Anti-PCNA Antibody (clone PC10) IHC-plus™ LS-B1887

Note: This antibody replaces LS-C41094

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PCNA Antibodies

Anti-PCNA Antibody (Internal) IHC-plus™ LS-B402
Rabbit Polyclonal to Human PCNA
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PCNA Antibody (C-Terminus) IHC-plus™ LS-B2826
Rabbit Polyclonal to Human PCNA
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-PCNA Antibody IHC-plus™ LS-B4230
Mouse Monoclonal (IgG2a) to Rat PCNA
Rat, Human, Insect, S. pombe, Vertebrate
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone PC10] (IgG2a) to Human PCNA
Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human PCNA
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [PC10]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry

Specificity and Use

PCNA antibody was raised against recombinant human PCNA.
Recombinant rat PCNA
Immunohistochemistry: LS-B1887 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1887 was determined to be 10 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Store at 4°C. Do not freeze.
For research use only.

About PCNA

P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Immunohistochemistry

Immunohistochemistry
Anti-PCNA antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1887 concentration 10 ug/ml.

Western blot

Western blot
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Requested From: United States
Date Requested: 12/10/2016

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