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| Catalog Number | Size | Price |
|---|---|---|
| LS-C287181-10 | 10 µl | $295 |
| LS-C287181-100 | 100 µl (1 mg/ml) | $514 |
![PCNA Antibody - Flow Cytometric Detection of PCNA v.DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. One million cells were stained with 0.03 ug anti-PCNA []. Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody [A120-114F], and DNA stained with PI.](https://lsbio-7d62.kxcdn.com/image2/pcna-antibody-aa75-125-ls-c287181/163714_1125621.jpg)
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Polyclonal Rabbit anti‑Human PCNA Antibody (aa75‑125, IHC, WB) LS‑C287181
Polyclonal Rabbit anti‑Human PCNA Antibody (aa75‑125, IHC, WB) LS‑C287181
Antibody:
PCNA Rabbit anti-Human Polyclonal (aa75-125) Antibody
Application:
IHC, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PCNA Rabbit anti-Human Polyclonal (aa75-125) Antibody
Application:
IHC, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
PCNA antibody LS-C287181 is an unconjugated rabbit polyclonal antibody to PCNA (aa75-125) from human. It is reactive with human and mouse. Validated for Flow, IHC, IP and WB.
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 5111 corresponding to amino acids between 75 and 125 of UniprotID: P12004
Epitope
aa75-125
Specificity
Region between residues 75 and 125 of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).
Applications
- IHC (1:2000 - 1:10000)
- Western blot (1:1000 - 1:10000)
- Immunoprecipitation (2 - 5 µg/mg lysate)
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Flo: 0.03 µg per 1 X 10^6 cells in a 150 µl volume
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Publications (0)
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Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Rat, Human, Mouse, Chicken, Zebrafish, Drosophila, Yeast, Primate
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat, Chicken, Xenopus
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
