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Anti-PCK1 Antibody (aa330-379) IHC-plus™ LS-B1555

Note: This antibody replaces LS-C30427

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PCK1 Antibodies

Anti-PCK1 Antibody (aa330-379) IHC-plus™ LS-B1555
Rabbit Polyclonal (IgG) to Human PCK1
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PCK1 Antibody (aa513-524) IHC-plus™ LS-B3032
Goat Polyclonal to Human PCK1
Human, Monkey, Bat, Dog, Horse, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PCK1 Antibody IHC-plus™ LS-B6935
Rabbit Polyclonal (IgG) to Mouse PCK1
Mouse, Human, Rat, Hamster
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PCK1
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit, Chicken
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PCK1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Bat, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PCK1 antibody was raised against synthetic peptide located between aa330-379 of human PCK1 (P35558, NP_002582). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Opossum, Turkey, Zebra finch, Chicken (100%); Bat, Pig, Platypus, Stickleback (92%); Salmon (91%); Dog, Guinea pig, Xenopus (85%).
LS-E11547 - Lyophilized - 100 µg - $145.00
aa330-379
Human PCK1
Immunohistochemistry: LS-B1555 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1555 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
Reconstitute with 50ul sterile ddH2O.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PCK1

P35558 NM_002591 NP_002582.3

PCK1 Antibody, PEPCKC Antibody, Phosphopyruvate carboxylase Antibody, PEP carboxykinase Antibody, PEPCK-C Antibody, PEPCK1 Antibody

PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency.

Immunohistochemistry

Immunohistochemistry
Anti-PCK1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1555 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-PCK1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1555 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
PCK1 antibody LS-B1555 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2016

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