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Anti-PCDH1 / PCD1 Antibody (aa710-971) LS-C186370

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.69 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Rabbit Polyclonal (IgG) to Human PCDH1 / PCD1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human PCDH1 / PCD1
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Bovine (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

PCDH1 / PCD1 antibody was raised against recombinant fragment corresponding to a region within amino acids 710 and 971 of PCDH1 (SwissProt Q08174). Percent identity by BLAST analysis: Human (100%); Bovine (98%); Mouse (97%).
aa710-971
Human PCDH1 / PCD1
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About PCDH1 / PCD1

Q08174 NM_002587 NP_002578.2

PCDH1 Antibody, Cadherin-like protein 1 Antibody, PC42 Antibody, Protocadherin-42 Antibody, Protocadherin 1 Antibody, Protocadherin-1 Antibody, PCDH42 Antibody, Protocadherin 42 Antibody, Cadherin-like 1 Antibody, PCD1 Antibody

PCDH1 / PCD1 belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded BT474 xenograft using PCDH1 antibody at 1:100 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HCT116. 7.5% SDS PAGE. PCDH1 / PCD1 antibody diluted at 1:1000.

Requested From: United States
Date Requested: 12/5/2016

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