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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PBX3 Antibody (clone 1A11) IHC‑plus™ LS‑B6292
Note: This antibody replaces LS-C133922
PBX3 antibody LS-B6292 is an unconjugated mouse monoclonal antibody to human PBX3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B6292-50
50 µg (0.5 mg/ml)
Unavailable

Popular PBX3 Products

PBX3 antibody LS-C31841 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Immunoperoxidase of monoclonal antibody to PBX3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.3 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PBX3 antibody LS-C31841 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Monkey, Mouse, Rat, Bovine, Guinea pig, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

PBX3 antibody LS-B6292 is an unconjugated mouse monoclonal antibody to human PBX3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About PBX3
Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. P40426 NM_006195 NP_006186.1

PBX3 Antibody, Pre-B-cell leukemia homeobox 3 Antibody, Homeobox protein PBX3 Antibody


Specifications

Target
Human PBX3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1A11]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PBX3 antibody was raised against pBX3 (NP_006186, 342 a.a. ~ 435 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Pbx3
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
MicroRNA profiling in pediatric pilocytic astrocytoma reveals biologically relevant targets, including PBX3, NFIB, and METAP2. Ho CY, Bar E, Giannini C, Marchionni L, Karajannis MA, Zagzag D, Gutmann DH, Eberhart CG, Rodriguez FJ. Neuro-oncology. 2013 15:69-82. (IHC-P; Human) [PubMed:23161775] [PMC:PMC3534421]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.
Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.

Western blot

LS-B6292 Western blot of PBX3 expression in K-562.
LS-B6292 Western blot of PBX3 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.
Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.

Western blot

LS-B6292 Western blot of PBX3 expression in K-562.
LS-B6292 Western blot of PBX3 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.
Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.

Western blot

LS-B6292 Western blot of PBX3 expression in K-562.
LS-B6292 Western blot of PBX3 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.
Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6292 concentration 5 ug/ml.

Western blot

LS-B6292 Western blot of PBX3 expression in K-562.
LS-B6292 Western blot of PBX3 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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