Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas.
PAX8 antibody detects PAX8 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody dilution:1:500.
Immunofluorescence of paraformaldehyde-fixed A549 using PAX8 antibody at 1:200 dilution.
Sample (30 ug whole cell lysate). A:293T. 10% SDS PAGE. PAX8 antibody diluted at 1:1000
PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChrIP experiments. ChrIP Sample:293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.
Requested From: United States
Date Requested: 1/16/2017