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Anti-PAX5 Antibody (clone 8F9) IHC-plus™ LS-B5491

Note: This antibody replaces LS-C133362

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PAX5 Antibodies

Anti-PAX5 Antibody (clone 3A7) LS-C175709
Mouse Monoclonal [clone 3A7] (IgG1) to Human PAX5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PAX5 Antibody (clone 1H9) IHC-plus™ LS-B11922
Rat Monoclonal [clone 1H9] (IgG2a,k) to Mouse PAX5
Mouse, Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8F9] (IgG1,k) to Human PAX5
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PAX5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8F9]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

PAX5 antibody was raised against pAX5 (NP_057953, 192 a.a. ~ 302 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PAX5
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.2. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About PAX5

Q02548 NM_016734 NP_057953.1

PAX5 Antibody, BSAP Antibody, Paired box 5 Antibody, Paired box protein Pax-5 Antibody, Transcription factor PAX 5 Antibody, Paired box homeotic gene 5 Antibody

PAX5 is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation.

Immunohistochemistry

Immunohistochemistry
Anti-PAX5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5491 concentration 5 ug/ml.

Western blot

Western blot
PAX5 monoclonal antibody clone 8F9. Western blot of PAX5 expression in IMR-32.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PAX5 is approximately 0.03 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/2/2016

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