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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAX5 Antibody (aa2‑14) LS‑C34648
PAX5 antibody LS-C34648 is an unconjugated goat polyclonal antibody to PAX5 from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C34648-100
100 µg (0.5 mg/ml)
Unavailable

Popular PAX5 Products

Surface staining of C57Bl/6 splenocytes with PE-Cy7 anti-mouse CD23 (LS-C107404) and FITC anti-mouse CD21/35 followed by intracellular staining using Foxp3 Buffers and 0.125 ug of PE anti-mouse/human Pax5. Histogram depicts cell populations based on surface stains comprising the marginal zone cells (CD21high, CD23-, blue histogram) or T1 cells (CD21-, CD23-, green histogram). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: Flow Cytometry
Anti-PAX5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5491 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded Human bladder tissue using anti-PAX5 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-PAX5 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10740 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot

Product Description

PAX5 antibody LS-C34648 is an unconjugated goat polyclonal antibody to PAX5 from human and monkey. Validated for WB.
About PAX5
PAX5 is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. Q02548 NM_016734 NP_057953.1

PAX5 Antibody, BSAP Antibody, Paired box 5 Antibody, Paired box protein Pax-5 Antibody, Transcription factor PAX 5 Antibody, Paired box homeotic gene 5 Antibody


Specifications

Target
Human PAX5
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (2 - 4 µg/ml)
Failed Applications
  • IHC - Paraffin
Immunogen
PAX5 antibody was raised against synthetic peptide DLEKNYPTPRTSR-C from the N-terminus of human PAX5 (NP_057953.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant (100%); Mouse, Rabbit, Pig (92%); Dog, Horse (85%).
Blocking Peptide
LS-E25404 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C34648. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-14
Specificity
Human PAX5.
Usage
Western blot: Approx 50 kDa band seen in U937 lysate (predicted MW 45 kDa). Recommended for use at 2-4 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
PAX5 antibody staining (3 ug/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/22/2018

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