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Anti-PAX2 Antibody (phospho-Ser393) IHC-plus™ LS-B2450

Note: This antibody replaces LS-C9503


Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular PAX2 Antibodies

Anti-PAX2 Antibody (aa188-385) LS-C9505
Rabbit Polyclonal (IgG) to Mouse PAX2
Mouse, Human, Rat, Xenopus
IHC, Western blot, Immunoprecipitation, ELISA
Anti-PAX2 Antibody (aa174-223) LS-C31501
Rabbit Polyclonal (IgG) to Human PAX2
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Zebrafish
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-PAX2 Antibody (clone 3C7) IHC-plus™ LS-B4389
Mouse Monoclonal [clone 3C7] (IgG2a,k) to Human PAX2
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PAX2
IHC - Paraffin, Western blot, ELISA


Human PAX2
Human (tested or 100% immunogen sequence identity)
Mouse, Chicken, Frog, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (1:50)
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

PAX2 antibody was raised against synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2).
Recognizes human Pax2 protein when phosphorylated at Ser393. Based on amino acid sequence homology with Pax5 and Pax8, cross-reactivity with these proteins is likely. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK. Species sequence Homology: mouse, chicken, zebra sh and frog - 100%.
Immunohistochemistry: LS-B2450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2450 was determined to be 1:50. Western Blot: Analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8.


PBS, pH 7.4, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About PAX2

Q02962 NM_003987 NP_003978.2

PAX2 Antibody, Paired box homeotic gene 2 Antibody, Paired box protein Pax-2 Antibody, PAPRS Antibody, Paired box 2 Antibody, Paired box gene 2 Antibody

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.


Anti-PAX2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2450 dilution 1:50.

Requested From: United States
Date Requested: 10/21/2016

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