Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
PAX2 antibody was raised against a GST-Pax-2 fusion protein derived from the C-terminal domain (aa188-385) of the murine Pax-2 protein (1).
Recognizes Pax-2 protein and does not cross-react with other Pax family members. The major epitopes are located between aa204-373 of mouse Pax-2. Two proteins are detected in mouse embryonic kidney lysates with this antibody: a major band at 46kD (Pax-2b) and a minor band at 48kD (Pax-2a). Reactivity: ELISA: Immunogen; WB: Xenopus, zebrafish, embryonic mouse kidney, human Wilms' tumors; IHC (frozen): Rat, Xenopus, zebrafish, human Wilms' tumor, mouse; IHC (paraffin): Human Wilms' tumor, mouse; IP: Embryonic mouse kidney.
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 1 ug/ml. Immunohistochemistry: 5 ug/ml (whole mount staining).
PBS, pH 7.4, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.
Hyperoxia causes reduced density of retinal astrocytes in the central avascular zone in the mouse model of oxygen-induced retinopathy. Bucher F, Stahl A, Agostini HT, Martin G. Molecular and cellular neurosciences. 2013 Sep;56:225-33. [Full Text Article]