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Anti-PARP1 Antibody (N-Terminus) IHC-plus™ LS-B1243

Note: This antibody replaces LS-C32851
Catalog Size Price
LS-B1243-50 50 µg (0.2 mg/ml) Unavailable

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Anti-PARP1 Antibody (clone A6.4.12) IHC-plus™ LS-B3432
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Anti-PARP1 Antibody IHC-plus™ LS-B5181
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Anti-PARP1 Antibody (Gly623) IHC-plus™ LS-B5991
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100% Guaranteed
Rabbit Polyclonal to Human PARP1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human PARP1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

PARP1 antibody was raised against synthetic peptide from human PARP1.
N-Terminus
Peptide from the N-terminus of human PARP1
Immunohistochemistry: LS-B1243 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1243 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
+4°C, avoid freezing
For research use only.

About PARP1

P09874 NM_001618 NP_001609.2

PARP1 Antibody, ADPRT Antibody, ADP-ribosyltransferase NAD(+) Antibody, Adp-ribosyltransferase Antibody, ADPRT1 Antibody, ARTD1 Antibody, ADPRT 1 Antibody, Poly(ADP-ribose) synthetase Antibody, Poly(ADP-ribosyl)transferase Antibody, PARP Antibody, Poly [ADP-ribose] polymerase 1 Antibody, PADPRT-1 Antibody, PARP-1 Antibody, Poly (ADP-ribose) polymerase 1 Antibody, Poly(ADP-ribose) polymerase Antibody, Poly[ADP-ribose] synthase 1 Antibody, PPOL Antibody

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR.

Immunohistochemistry

Immunohistochemistry
Anti-PARP antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1243 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-PARP antibody IHC of human brain, cerebellum, white matter. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1243 concentration 10 ug/ml.

Requested From: 
Date Requested: 6/23/2017

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