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Anti-PARP1 Antibody (aa1-1014) IHC-plus™ LS-B8446

Note: This antibody replaces LS-C9484

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PARP1 Antibodies

Anti-PARP1 Antibody (N-Terminus) LS-C17208
Rabbit Polyclonal (IgG) to Human PARP1
Human, Bovine
IHC, Western blot
Unconjugated
Anti-PARP1 Antibody (clone A6.4.12) IHC-plus™ LS-B3432
Mouse Monoclonal [clone A6.4.12] (IgG1) to Human PARP1
Human, Mouse, Rat, Hamster, Xenopus, Drosophila
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PARP1 Antibody (clone 10H) LS-C146823
Mouse Monoclonal [clone 10H] (IgG3,k)
ICC, Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PARP1
Human, Mouse, Bovine, Chicken, Xenopus
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human PARP1
Rabbit
Human, Mouse, Bovine, Chicken, Xenopus (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • ICC (1:2000)
  • Western blot (1:2000)
  • Immunoprecipitation (1:200)
  • ELISA

Specificity and Use

PARP1 antibody was raised against recombinant human PARP-1 (poly(ADP-ribose) polymerase-1) (aa 1-1014) produced in Sf9 cells.
aa1-1014
Recognizes human PARP-1. Detects bands of ~116kD (PARP-1) and ~85kD (apoptosis-induced cleavage fragment) by Western Blot. Does not cross-react with PARP-2. Species cross-reactivity: mouse, bovine, chicken and xenopus.
Suitable for use in ELISA, Immunocytochemistry. Immunohistochemistry, Immunoprecipitation and Western Blot. Immunocytochemistry: 1:2000. Immunohistochemistry: Frozen and paraffin sections. Immunoprecipitation: 1:200. Western Blot: 1:2000.

Packaging

0.09% sodium azide
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
For research use only.

About PARP1

P09874 NM_001618 NP_001609.2

PARP1 Antibody, ADPRT Antibody, ADP-ribosyltransferase NAD(+) Antibody, Adp-ribosyltransferase Antibody, ADPRT1 Antibody, ARTD1 Antibody, ADPRT 1 Antibody, Poly(ADP-ribose) synthetase Antibody, Poly(ADP-ribosyl)transferase Antibody, PARP Antibody, Poly [ADP-ribose] polymerase 1 Antibody, PADPRT-1 Antibody, PARP-1 Antibody, Poly (ADP-ribose) polymerase 1 Antibody, Poly(ADP-ribose) polymerase Antibody, Poly[ADP-ribose] synthase 1 Antibody, PPOL Antibody

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR.

Immunohistochemistry

Immunohistochemistry
Anti-PARP1 / PARP antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8446 dilution 1:100.
 

Requested From: United States
Date Requested: 12/4/2016

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