Human PARK7 / DJ-1

Rabbit

Human, Chimpanzee, Zebrafish, African green monkey (tested or 100% immunogen sequence identity)

Chimpanzee, Zebrafish, African green monkey (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of Human PARK7 protein.

C-Terminus

Cross reactivity with PARK7 from frog, mouse, rat, dog, chicken, Japanese rice fish and Atlantic salmon may also occur as the sequence varies by only one amino acid residue as indicated by BLAST analysis. Reactivity with PARK7 protein from other sources is not known.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:10000 - 1:50000)

Immunohistochemistry: LS-B1452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1452 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99497 NCBI: NM_007262 NP_009193.2