Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
PARK7 / DJ-1 antibody was raised against a synthetic peptide mapping to the human PARK7 (DJ-1) protein, residues 167-189. AIVEALNGKEVAAQVKAPLVLKD. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Elephant (96%); Panda, Horse, Rabbit (91%); Hamster, Dog, Bat, Pig, Turkey, Chicken (87%); Mouse, Rat, Bovine, Salmon (83%).
Immunohistochemistry: LS-B266 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B266 was determined to be 2.5 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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