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Anti-PARK2 / Parkin 2 Antibody (Internal) LS-C2827

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $335
Inquire for larger quantities

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Rabbit Polyclonal to Human PARK2 / Parkin 2
Human
Western blot
Unconjugated

Details

Human PARK2 / Parkin 2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Purified IgG

Applications

Western blot (1:250 - 1:1000)

Specificity and Use

PARK2 / Parkin 2 antibody was raised against reacts with an internal sequence of the human 52 kD Parkin protein
Internal
Reacts with human protein. Other species not tested.

Packaging

Serum, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PARK2 / Parkin 2

O60260 NM_004562 NP_004553.2

PARK2 Antibody, AR-JP Antibody, LPRS2 Antibody, Parkin Antibody, PDJ Antibody, Parkinson disease protein 2 Antibody, PRKN Antibody

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context.

Western blot

Western blot
The Parkin antibody was tested to pull down endogenously expressed Parking in HIT-T15 cells (Lane 1, HIT-T15 cells; Lane 2, negative control) and transiently transfected Parkin in Hek cells (Lane 3, Hek cells; Lane 4, negative control). Amount of antibody used: 4 ul (=4 ug) Lysate of cells used: 1000 ug.

Western blot

Western blot
The Parkin antibody was tested to detect Parkin levels in stable Hek cells expressing Parkin. Antibody dilutions: 1:250 and 1:1000 Hek cell lysate used: 50 ug (lanes 1 & 4): 100 ug (lanes 2 & 5); 200 ug (lanes 3 & 6).

Requested From: United States
Date Requested: 9/25/2016

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