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Anti-PARK2 / Parkin 2 Antibody (aa111-140) LS-C99184

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Orders@LSBio.com
 

Most Popular PARK2 / Parkin 2 Antibodies

Anti-PARK2 / Parkin 2 Antibody (aa111-140) LS-C99184
Rabbit Polyclonal to Human PARK2 / Parkin 2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-PARK2 / Parkin 2 Antibody (aa23-416) LS-C357496
Rabbit Polyclonal (IgG) to Human PARK2 / Parkin 2
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human PARK2 / Parkin 2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human PARK2 / Parkin 2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

PARK2 / Parkin 2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 111-140 from N-terminal region of human Parkin.
LS-E8222 - Lyophilized - 100 µg - $145.00
aa111-140

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Overexpression of parkin ameliorates dopaminergic neurodegeneration induced by 1- methyl-4-phenyl-1,2,3,6-tetrahydropyridine in mice. Bian M, Liu J, Hong X, Yu M, Huang Y, Sheng Z, Fei J, Huang F. PloS one. 2012 7:e39953. [PubMed:22792139] [PMC:PMC3390003]

About PARK2 / Parkin 2

O60260 NM_004562 NP_004553.2

PARK2 Antibody, AR-JP Antibody, LPRS2 Antibody, Parkin Antibody, PDJ Antibody, Parkinson disease protein 2 Antibody, PRKN Antibody

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context.

Immunohistochemistry

Immunohistochemistry
Parkin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of Parkin Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of lysate from SH-SY5Y cell line, using Park2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Flow Cytometry

Flow Cytometry
Parkin Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/6/2016

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