Human PAPPA / PAPP-A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

Unconjugated

Protein A/G purified

Unmodified

Purified human PAPP-A.

Recognizes native Pregnancy-associated plasma protein A (human, PAPP-A) Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. The epitope of this product is different from those of clones 6F172 and 6F173. The epitope is located on the PAPP-A subunit of the heterotetramer. Reactivity: In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. This product detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.

• IHC
• Western blot
• ELISA (1:8000)

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. Western Blot: Detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.

PBS, pH 7.2, 15 mM Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13219 NCBI: NM_002581 NP_002572.2