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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAPPA / PAPP‑A Antibody LS‑C9691
Antibody
PAPP-A antibody LS-C9691 is an unconjugated mouse monoclonal antibody to human PAPP-A (PAPPA). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C9691-100
100 µl
Unavailable

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Product Description

PAPP-A antibody LS-C9691 is an unconjugated mouse monoclonal antibody to human PAPP-A (PAPPA). Validated for ELISA, IHC and WB.
About PAPPA / PAPP-A
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Q13219 NM_002581 NP_002572.2

PAPPA Antibody, DIPLA1 Antibody, IGF-dependent IGFBP-4 protease Antibody, PAPPA1 Antibody, PAPA Antibody, PAPP-A Antibody, Pappalysin-1 Antibody, ASBABP2 Antibody, IGFBP-4ase Antibody


Specifications

Target
Human PAPPA / PAPP-A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PAPPA / PAPP-A antibody was raised against purified human PAPP-A.
Specificity
Recognizes native Pregnancy-associated plasma protein A (human, PAPP-A) Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. The epitope of this product is different from those of clones 6F172 and 6F173. The epitope is located on the PAPP-A subunit of the heterotetramer. Reactivity: In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. This product detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.
Usage
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination is this product used as coating antibody and biotinylated clone 6F173 used as detection antibody. This product can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. Western Blot: Detects both reduced and unreduced PAPP-A in Western Blot, though reaction to unreduced PAPP-A is less than that of clones 6F172 and 6F173.
Presentation
PBS, pH 7.2, 15 mM sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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