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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAPPA / PAPP‑A Antibody (clone 1_24) LS‑C9690
PAPP-A antibody LS-C9690 is an unconjugated mouse monoclonal antibody to human PAPP-A (PAPPA). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C9690-100
100 µl
$495
Description
PAPP-A antibody LS-C9690 is an unconjugated mouse monoclonal antibody to human PAPP-A (PAPPA). Validated for ELISA, IHC and WB.
Target
Human PAPPA / PAPP-A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1_24]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PAPPA / PAPP-A antibody was raised against purified human PAPP-A.
Specificity
Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid-treated PAPP-A. The epitope is different from those of clones 6F172 and 6F174 and is located on the PAPP-A subunit of the heterotetramer. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. This product detects only unreduced PAPP-A in Western Blot. It gives the strongest signal in Immunohistochemistry compared to clone 6F172 and clone 6F174.
Usage
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. Western Blot: Detects only unreduced PAPP-A. Immunohistochemistry: Gives the strongest signal compared to clone 6F172 and clone 6F174.
Presentation
PBS, pH 7.2, 15 mM Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About PAPPA / PAPP-A
Q13219 NM_002581 NP_002572.2

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Requested From: United States
Date Requested: 12/15/2018
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