Human PAPPA / PAPP-A
(tested or 100% immunogen sequence identity)
Protein A/G purified
- Western blot
- ELISA (1:8000)
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PAPPA / PAPP-A antibody was raised against purified human PAPP-A.
Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid-treated PAPP-A. The epitope is different from those of clones 6F172 and 6F174 and is located on the PAPP-A subunit of the heterotetramer. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. This product detects only unreduced PAPP-A in Western Blot. It gives the strongest signal in Immunohistochemistry compared to clone 6F172 and clone 6F174.
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. ELISA: 1:8000. In sandwich ELISA the optimal combination was clone 6F174 as coating and biotinylated clone 6F173 as detection. Western Blot: Detects only unreduced PAPP-A. Immunohistochemistry: Gives the strongest signal compared to clone 6F172 and clone 6F174.
PBS, pH 7.2, 15 mM Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.