PAO antibody LS-C82771 is an unconjugated rabbit polyclonal antibody to PAO (PAOX) from human, rat, horse and other species. Validated for WB.
Human, Monkey, Rat, Horse
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
PAOX / PAO antibody was raised against synthetic peptide from C-Terminus of human PAOX (Q5VWY0, NP_690875). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Rat, Elephant, Horse, Rice (100%); Mouse, Panda, Bovine, Bat, Rabbit, Opossum, Guinea pig, Zebra finch (92%); Chicken, Xenopus, Stickleback, Zebrafish (91%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.