Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human PAK1 + PAK2 + PAK3
Human, Mouse, Rat, Guinea pig
Human PAK1 + PAK2 + PAK3
Human, Mouse, Rat, Guinea pig (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:1000)
Specificity and Use
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr423 of human PAK1.
Detects endogenous human PAK1, PAK2 and PAK3 only when phosphorylated at Thr423, Thr402 and Thr421, respectively. Does not cross-react with phosphorylated PAK4, PAK5 or PAK6. Does cross-react with phospho-Mst1 (Thr183) or phospho-Mst2 (Thr180). Species cross-reactivity: Guinea pig. Predicted to cross-react with mouse and rat based on sequence homology.
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.