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Anti-PADI4 / PAD4 Antibody (clone 4H5) LS-C173989

Catalog	Size	Price
LS-C173989-100	100 µl (1 mg/ml)	Unavailable

Related Products

27 PADI4 / PAD4 Antibodies

Most Popular PADI4 / PAD4 Antibodies

Anti-PADI4 / PAD4 Antibody (aa398-447) LS-C81955

Antibody:	Rabbit Polyclonal (IgG) to Human PADI4 / PAD4
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Rabbit, Chicken
Application:	Western blot
Format:	Unconjugated

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-PADI4 / PAD4 Antibody (C-Terminus) LS-C144651

Antibody:	Rabbit Polyclonal to Human PADI4 / PAD4
Reactivity:	Human, Mouse, Rat
Application:	IHC, Western blot, Immunoprecipitation, ELISA
Format:	Unconjugated

Anti-PADI4 / PAD4 Antibody (C-Terminus) LS-C153535

Antibody:	Rabbit Polyclonal to Human PADI4 / PAD4
Reactivity:	Human, Mouse, Rat
Application:	IHC, Western blot, Immunoprecipitation, ELISA
Format:	Unconjugated

Anti-PADI4 / PAD4 Antibody (clone 5E7) LS-C173990

Antibody:	Mouse Monoclonal [clone 5E7] (IgG1) to Human PADI4 / PAD4
Reactivity:	Human
Application:	IHC, IHC - Paraffin, Western blot
Format:	Unconjugated

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody.

Anti-PADI4 / PAD4 Antibody (clone 5B7) LS-C174036

Antibody:	Mouse Monoclonal [clone 5B7] (IgG2a) to Human PADI4 / PAD4
Reactivity:	Human
Application:	Western blot, Flow Cytometry
Format:	Unconjugated

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.

Antibody:	Mouse Monoclonal [clone 4H5] (IgG2a) to Human PADI4 / PAD4
Reactivity:	Human
Application:	IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Format:	Unconjugated

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Human pancreas tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Human breast tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Human prostate tissue using anti-PADI4 mouse monoclonal antibody.

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PADI4 mouse monoclonal antibody.

Anti-PADI4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4.

Flow cytometry of Jurkat cells, using anti-PADI4 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow cytometry of HeLa cells, using anti-PADI4 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Details

Target:	Human PADI4 / PAD4
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG2a Monoclonal [4H5]
Conjugations:	Unconjugated
Purification:	Protein A/G purified
Modifications:	Unmodified

Applications

Approved:

IHC
IHC - Paraffin (1:150)
Immunofluorescence (1:100)
Western blot (1:2000)
Flow Cytometry (1:100)

Specificity and Use

Immunogen:	PADI4 / PAD4 antibody was raised against full length human recombinant protein of human PADI4(NP_036519) produced in HEK293T cell.
Specificity:	Human PADI4 / PAD4

Packaging

Presentation:	PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About PADI4 / PAD4

Uniprot: Q9UM07 NCBI: NM_012387 NP_036519.2

Synonyms: PADI4 Antibody, HL-60 PAD Antibody, PAD4 Antibody, PAD Antibody, PDI5 Antibody, PDI4 Antibody, PADI-H protein Antibody, PADI5 Antibody, Peptidylarginine deiminase IV Antibody

Description: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci).

(More About PADI4 / PAD4)

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Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human breast tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PADI4 mouse monoclonal antibody.

Immunofluorescence

Anti-PADI4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-PADI4 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-PADI4 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Requested From:
Date Requested: 4/23/2018

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