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Anti-p66 / SHC Antibody (Tyr349) IHC-plus™ LS-B1489

Note: This antibody replaces LS-C34488

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular p66 / SHC Antibodies

Anti-p66 / SHC Antibody (clone 3F4) IHC-plus™ LS-B4416
Mouse Monoclonal [clone 3F4] (IgG,k) to Human p66 / SHC
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, Proximity Ligation Assay
Unconjugated
Immunohistochemistry Image
Anti-p66 / SHC Antibody (phospho-Tyr427) LS-C34237
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-p66 / SHC Antibody (phospho-Ser36, clone 6E10) LS-C10125
Mouse Monoclonal [clone 6E10] (IgG1) to Human p66 / SHC
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human p66 / SHC
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human p66 / SHC
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot

Specificity and Use

p66 / SHC antibody was raised against synthetic peptide from human SHC1.
Tyr349
Amino acids surrounding Tyr 349 of human SHC1
Immunohistochemistry: LS-B1489 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1489 was determined to be 10 ug/ml.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About p66 / SHC

P29353 NM_003029 NP_003020.2

SHC1 Antibody, p46 shc Antibody, p52 shc Antibody, SH2 domain protein C1 Antibody, p66 Antibody, SHCA Antibody, SHC Antibody, SHC-transforming protein 1 Antibody, SHC-transforming protein 3 Antibody, SHC-transforming protein A Antibody

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals.

Immunohistochemistry

Immunohistochemistry
Anti-SHC1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1489 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using Shc1(Ab-349) antibody.

Western blot

Western blot
Western blot of extracts from HepG2 cells using Shc1(Ab-349) antibody.
 

Requested From: United States
Date Requested: 12/9/2016

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