LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
P5CR2 / PYCR2 Antibody (clone 4A10) LS‑C115748
PYCR2 antibody LS-C115748 is an unconjugated mouse monoclonal antibody to human PYCR2 (P5CR2). Validated for Flow, IF and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C115748-100
100 µl (0.67 mg/ml)
Unavailable

Popular P5CR2 / PYCR2 Products

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
Applications: Western blot
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Applications: Western blot
Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Species: Human, Monkey
Applications: Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human colon tissue using anti-PYCR2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

PYCR2 antibody LS-C115748 is an unconjugated mouse monoclonal antibody to human PYCR2 (P5CR2). Validated for Flow, IF and WB. Cited in 1 publication.
About P5CR2 / PYCR2
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP+. Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Q96C36 NM_013328 NP_037460.2

PYCR2 Antibody, p5CR 2 Antibody, p5C reductase 2 Antibody, p5CR2 Antibody


Specifications

Target
Human P5CR2 / PYCR2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [4A10]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
P5CR2 / PYCR2 antibody was raised against full length human recombinant protein of human PYCR2 (NP_037460) produced in HEK293T cell.
Specificity
Human P5CR2 / PYCR2
Usage
Concentration: 0.67 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination. Nakayama T, Al-Maawali A, El-Quessny M, Rajab A, Khalil S, Stoler JM, Tan WH, Nasir R, Schmitz-Abe K, Hill RS, Partlow JN, Al-Saffar M, Servattalab S, LaCoursiere CM, Tambunan DE, Coulter ME, Elhosary PC, Gorski G, Barkovich AJ, Markianos K, Poduri A, Mochida GH. American journal of human genetics. 2015 96:709-19. (WB; Human) [Full Text Article] [PubMed:25865492] [PMC:PMC4570282]

Customer Reviews (0)


Images


Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of Jurkat cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-PYCR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCR2 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/18/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number