Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors.
Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody (M01), clone 3F7-F5.Lane 1: LCK transfected lysate(59.4 KDa).Lane 2: Non-transfected lysate.
LCK monoclonal antibody (M01), clone 3F7-F5 Western Blot analysis of LCK expression in Jurkat.
Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged LCK is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody (M01) clone 3F7-F5 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Requested From: United States
Date Requested: 10/27/2016