Human p56lck / LCK

Rabbit

Human, Mouse, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

GST fusion protein corresponding to aa1-58 of human Lck.

aa1-58

Recognizes Lck, Mr 56kD of mouse, human and bovine; reactivity with other Src family members not determined.

• Western blot
• Immunoprecipitation

Western Blot Analysis: 0.5-2 ug/ml detected Lck in RIPA lysates from Jurkat cells. Included Positive Antigen Control: Jurkat cell lysate. Use 20 ug per lane for minigels. Immunoprecipitation: 4 ug immunoprecipitated Lck from 1 ug of Jurkat RIPA lysate as confirmed by subsequent immunoblot using monoclonal anti-Lck. Western Blot Analysis Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lck (1 ug/ml). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P06239 NCBI: NM_005356 NP_005347.3