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Anti-P450SCC / CYP11A1 Antibody (aa288-337) IHC-plus™ LS-B3997

Note: This antibody replaces LS-C81675

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular P450SCC / CYP11A1 Antibodies

Anti-P450SCC / CYP11A1 Antibody (aa288-337) IHC-plus™ LS-B3997
Rabbit Polyclonal (IgG) to Human P450SCC / CYP11A1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-P450SCC / CYP11A1 Antibody (aa507-521) LS-C312850
Rabbit Polyclonal (IgG) to Human P450SCC / CYP11A1
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image
Anti-P450SCC / CYP11A1 Antibody (aa391-515) LS-C374757
Rabbit Polyclonal (IgG) to Bovine P450SCC / CYP11A1
Bovine
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human P450SCC / CYP11A1
Human
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human P450SCC / CYP11A1
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

P450SCC / CYP11A1 antibody was raised against synthetic peptide located between aa288-337 of human CYP11A1 (P05108, NP_000772). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Monkey (92%).
LS-E18623 - Lyophilized - 100 µg - $145.00
aa288-337
Human CYP11A1
Immunohistochemistry: LS-B3997 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3997 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Steroidogenic enzyme expression in the human fetal liver and potential role in the endocrinology of pregnancy. O'Shaughnessy PJ, Monteiro A, Bhattacharya S, Fraser MJ, Fowler PA. Molecular human reproduction. 2013 19:177-87. (WB; Human) [PubMed:23197595]

About P450SCC / CYP11A1

P05108 NM_000781 NP_000772.2

CYP11A1 Antibody, Cholesterol 20-22 desmolase Antibody, Cholesterol desmolase Antibody, Cytochrome P450 11A1 Antibody, Cytochrome P450C11A1 Antibody, CYP11A Antibody, Cytochrome P450(scc) Antibody, p450SCC Antibody, Steroid 20-22-lyase Antibody, CYPXIA1 Antibody

P450SCC / CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones.

Immunohistochemistry

Immunohistochemistry
Anti-CYP11A1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3997 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-CYP11A1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3997 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-CYP11A1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3997 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/11/2016

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