Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

P40PHOX / NCF4

neutrophil cytosolic factor 4, 40kDa

P40PHOX / NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Name: neutrophil cytosolic factor 4, 40kDa
Synonyms: NCF4, SH3PXD4, NCF, Neutrophil cytosol factor 4, p40-phox, p40PHOX, NCF-4
Target Sequences: NM_013416 NP_038202.2 Q15080

Publications (1)

1
DPI Selectively Inhibits Intracellular NADPH Oxidase Activity in Human Neutrophils. Alicia Buck, Felix P Sanchez Klose, Vignesh Venkatakrishnan, Arsham Khamzeh, Claes Dahlgren, Karin Christenson, Johan Bylund. The Breast : official journal of the European Society of Mastology. 2019 Oct;3:488-497. [Full Text Article] [PubMed:31628159]

☰ Filters
Antibodies (65)
Proteins (10)
Over-Expression Lysate (4)
Primary (65)
Recombinant (6)
P40PHOX / NCF4 (75)
Human (57)
Mouse (20)
Rat (12)
Bovine (3)
Dog (3)
Gibbon (6)
Guinea pig (6)
Horse (7)
Monkey (7)
Pig (1)
Rabbit (4)
IHC (17)
IHC-P (5)
WB (55)
Flo (1)
ELISA (5)
IF (3)
Peptide-ELISA (4)
rabbit (47)
mouse (17)
goat (1)
Product Group
IHCPlus (1)
IgG (24)
IgG1 (14)
IgG2a (2)
IgG2a,k (1)
monoclonal mc (17)
polyclonal pc (48)
3C10 (1)
OTI12H10 (2)
OTI1A7 (2)
OTI2D4 (2)
OTI3E3 (2)
OTI3E8 (2)
OTI4B5 (2)
OTI4E5 (2)
OTI6H9 (2)
APC (2)
APC Conjugated (3)
Biotin Conjugated (6)
Carrier-free (8)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (6)
HRP Conjugated (6)
PE Conjugated (3)
Unconjugated (37)
aa111-339 (8)
aa132-339 (8)
aa216-265 (4)
aa267-316 (4)
aa51-100 (4)
pThr154 (2)
90-170 aa (1)
Arg149 (1)
C-Terminus (1)
Internal (1)
aa120-169 (1)
aa228-238 (1)
aa260-289 (1)
No (74)
Yes (1)
GST (1)
GST, N-terminus (1)
His (1)
His-T7 (2)
Myc-DDK (Flag) (5)
Human (7)
Mouse (1)
293T Cells (2)
E. coli (3)
HEK 293 Cells (3)
Wheat Germ Extract (2)
Purified (2)
P40PHOX / NCF4 Antibody - Immunofluorescence analysis of U20S cells.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
P40PHOX / NCF4 Antibody - Anti-NCF4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Select
P40PHOX / NCF4 Goat anti-Human Polyclonal (aa228-238) Antibody
Horse, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
P40PHOX / NCF4 Antibody - Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (aa260-289) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
P40PHOX / NCF4 Antibody - 1:100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
P40PHOX / NCF4 Protein
Select
E. coli
His
100 µg/$411; 500 µg/$985; 20 µg/$276
P40PHOX / NCF4 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NCF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (OTI4B5) Antibody
Human
IHC, WB
Unconjugated
100 µl/$434
P40PHOX / NCF4 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NCF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI4B5) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
P40PHOX / NCF4 Antibody - Immunohistochemistry of paraffin-embedded Rat brain using NCF4 Polyclonal Antibody at dilution of 1:100 (40x lens).
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
P40PHOX / NCF4 Antibody - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NCF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (OTI12H10) Antibody
Human
IHC
Unconjugated
100 µl/$434
P40PHOX / NCF4 Antibody - Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NCF4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:500)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI12H10) Antibody
Human
IHC
Unconjugated, Carrier-free
100 µg/$511
P40PHOX / NCF4 Antibody - Immunohistochemical analysis of p40 phox staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (Internal) Antibody
Rabbit, Pig, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$299
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (90-170 aa) Antibody
Mouse, Human
ELISA, IHC
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
P40PHOX / NCF4 Antibody - Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is blocked with the phospho peptide.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (pThr154) Antibody
Mouse, Human
IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
P40PHOX / NCF4 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human ovary tissue using p40 phox antibody.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
100 µg/$357
P40PHOX / NCF4 Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human ovary tissue using p40 phox (Phospho-Thr154) antibody.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (pThr154) Antibody
Mouse, Human
ELISA, IHC
Unconjugated
100 µg/$357
P40PHOX / NCF4 Antibody - Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using p40 phox Antibody. The picture on the right is blocked with the synthesized peptide.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (aa120-169) Antibody
Mouse, Human
IHC, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
P40PHOX / NCF4 Antibody - IHC of p40 phox (R149) pAb in paraffin-embedded human ovary tissue.
Select
P40PHOX / NCF4 Rabbit anti-Human Polyclonal (Arg149) Antibody
Mouse, Human
IHC, IHC-P
Unconjugated
50 µl/$321; 100 µl/$403
P40PHOX / NCF4 Antibody - Detection limit for recombinant GST tagged NCF4 is approximately 0.3 ng/ml as a capture antibody.
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (3C10) Antibody
Human
ELISA
Unconjugated
100 µg/$423
P40PHOX / NCF4 Antibody - Western blot of recombinant P40PHOX / NCF4.
Select
P40PHOX / NCF4 Rabbit anti-Mouse Polyclonal (aa111-339) Antibody
Mouse
WB
Unconjugated
100 µl/$301; 200 µl/$340; 500 µl/$470; 1 ml/$535; 2 ml/$698; 5 ml/$1,161
P40PHOX / NCF4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY NCF4. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCF4. (1:2000)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI3E3) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
P40PHOX / NCF4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY NCF4. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCF4. (1:500)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI3E8) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
P40PHOX / NCF4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY NCF4. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCF4. (1:2000)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI6H9) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
P40PHOX / NCF4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY NCF4. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCF4. (1:2000)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI2D4) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
P40PHOX / NCF4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY NCF4. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCF4. (1:2000)
Select
P40PHOX / NCF4 Mouse anti-Human Monoclonal (Carrier-free) (OTI1A7) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
Viewing 1-25 of 75 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).