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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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P40PHOX / NCF4 Antibody (C‑Terminus) LS‑C9419
NCF4 antibody LS-C9419 is an unconjugated rabbit polyclonal antibody to NCF4 (P40PHOX) from human and rat. Validated for WB.
Antibody
Catalog
Size
Price
LS-C9419-200
200 µg
$665

Popular P40PHOX / NCF4 Products

Immunofluorescence analysis of U20S cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Anti-NCF4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3604 concentration 2.5 ug/ml.
Species: Human, Monkey, Horse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Western blot analysis of extracts of various cell lines, using NCF4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 40s.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry
Western blot of recombinant P40PHOX / NCF4.
Species: Mouse
Applications: IHC, Western blot

Product Description

NCF4 antibody LS-C9419 is an unconjugated rabbit polyclonal antibody to NCF4 (P40PHOX) from human and rat. Validated for WB.
About P40PHOX / NCF4
P40PHOX / NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. Q15080 NM_013416 NP_038202.2

NCF4 Antibody, SH3PXD4 Antibody, NCF Antibody, Neutrophil cytosol factor 4 Antibody, p40-phox Antibody, p40PHOX Antibody, NCF-4 Antibody


Specifications

Target
Human P40PHOX / NCF4
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
P40PHOX / NCF4 antibody was raised against c-terminal 6HIS-tagged fusion protein corresponding to full length human p40 phox.
Epitope
C-Terminus
Specificity
Recognizes human p40 phox, Mr ~40kD. Species cross-reactivity: rat.
Usage
Suitable for use in Western Blot. Western Blot: 1 ug/ml detects p40 phox in RIPA lysates of HL-60 cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/22/2018

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