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Anti-P2RX3 / P2X3 Antibody (aa383-397) LS-C25959

Note: This antibody replaces LS-C91221, LS-C91222, LS-C91220, LS-C25958, LS-C91219

Ordering

Wt. Vol. Conc. Price
- 50 µl - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular P2RX3 / P2X3 Antibodies

Anti-P2RX3 / P2X3 Antibody (C-Terminus) IHC-plus™ LS-A9584
Rabbit Polyclonal to Human P2RX3 / P2X3
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Rat P2RX3 / P2X3
Rat, Mouse, Hamster
IHC, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Rat P2RX3 / P2X3
Rabbit
Rat, Mouse, Hamster (tested or 100% immunogen sequence identity)
Bovine, Dog, Horse (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC
  • ICC (1:500)
  • Immunofluorescence
  • Western blot (1:500)

Specificity and Use

P2RX3 / P2X3 antibody was raised against synthetic peptide corresponding to aa383-397 (VEKQSTDSGAYSIGH) of rat P2X3. Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Elephant, Panda, Bovine, Dog, Horse (93%); Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Rabbit, Opossum (87%).
aa383-397
Recognizes rat P2X3 Receptor. Species cross-reactivity: Human and primate.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Membranes were rinsed with PBS and incubated with Goat anti-rat IgG labeled with HRP for 1 hour at RT. Blocking buffer was removed. Slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times with PBS. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times with PBS and coverslipped. Staining was examined using Fluorescence Microscopy. Immunocytochemistry: 1:500. P2X3 transfected cells were processed for Indirect Immunofluorescence. Media was removed. Cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for Indirect Immunofluorescence as described for Immunohistochemistry.

Packaging

PBS, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About P2RX3 / P2X3

P56373 NM_002559 NP_002550.2

P2RX3 Antibody, ATP receptor Antibody, p2X3 Antibody, p2X3 purinoceptor Antibody, p2X purinoceptor 3 Antibody, Purinergic receptor P2X3 Antibody, Purinoceptor P2X3 Antibody, Purinergic receptor Antibody, p2RX3 Antibody, p2X receptor 3 Antibody, p2X receptor, subunit 3 Antibody

P2RX3 / P2X3 belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes.

Requested From: United States
Date Requested: 12/8/2016

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