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Anti-P2RX2 / P2X2 Antibody (aa460-472) LS-C25956

Note: This antibody replaces LS-C91218, LS-C91217

Ordering

Wt. Vol. Conc. Price
- 50 µl - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular P2RX2 / P2X2 Antibodies

Anti-P2RX2 / P2X2 Antibody (C-Terminus) LS-C25954
Guinea pig Polyclonal (IgG) to Rat P2RX2 / P2X2
Rat, Human
IHC, ICC, Western blot
Unconjugated
Anti-P2RX2 / P2X2 Antibody (aa55-104) IHC-plus™ LS-B990
Rabbit Polyclonal to Human P2RX2 / P2X2
Human, Monkey, Mouse, Rat, Guinea pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-P2RX2 / P2X2 Antibody (aa108-157) IHC-plus™ LS-B6608
Rabbit Polyclonal to Human P2RX2 / P2X2
Human, Monkey, Guinea pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Guinea pig Polyclonal (IgG) to Rat P2RX2 / P2X2
Rat, Mouse
IHC, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Rat P2RX2 / P2X2
Guinea pig
Rat, Mouse (tested or 100% immunogen sequence identity)
Guinea pig, Hamster (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC (1:500)
  • ICC (1:500)
  • Immunofluorescence
  • Western blot (1:500)

Specificity and Use

P2RX2 / P2X2 antibody was raised against synthetic peptide corresponding to aa460-472 (DSTSTDPKGLAQL) from the C-terminus of the rat P2X2. Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster, Guinea pig (92%); Human, Gorilla, Marmoset, Bat, Rabbit (85%).
aa460-472
Recognizes P2X2 Receptor. Species cross-reactivity: Rat and human.
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 1:500. Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8?C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated and diluted 1:500 with same buffer overnight at 2-8?C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence. Immunohistochemistry: 1:500. Male Sprague-Dawley rats (b. wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 ml of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8?C for 18-24 hours with antibody (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. Immunocytochemistry: 1:500. 2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above.

Packaging

PBS, 0.05% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About P2RX2 / P2X2

Q9UBL9 NM_012226 NP_036358.2

P2RX2 Antibody, ATP receptor Antibody, p2X2 Antibody, p2X Receptor, subunit 2 Antibody, Purinergic receptor Antibody, p2RX2 Antibody, p2X receptor 2 Antibody, p2X purinoceptor 2 Antibody, p2X2 purinoceptor Antibody

Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K+ recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.

Requested From: United States
Date Requested: 12/3/2016

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