Rat P2RX2 / P2X2

Guinea pig

Rat, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Synthetic peptide corresponding to aa460-472 (DSTSTDPKGLAQL) from the C-Terminus of rat P2X2. Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster, Guinea pig (92%); Human, Gorilla, Marmoset, Bat, Rabbit (85%).

aa460-472

Recognizes P2X2 Receptor. Species cross-reactivity: Rat and human.

• IHC (1:500)
• ICC (1:500)
• Immunofluorescence
• Western blot (1:500)

Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 1:500. Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8?C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated and diluted 1:500 with same buffer overnight at 2-8?C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence. Immunohistochemistry: 1:500. Male Sprague-Dawley rats (b. wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 ml of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8?C for 18-24 hours with antibody (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. Immunocytochemistry: 1:500. 2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above.

PBS, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UBL9 NCBI: NM_012226 NP_036358.2