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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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OXCT1 Antibody (aa316‑365) LS‑C81248
OXCT1 antibody LS-C81248 is an unconjugated rabbit polyclonal antibody to OXCT1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C81248-50
50 µg
Unavailable

Popular OXCT1 Products

Western blot of OXCT1 antibody.
Species: Human, Rat
Applications: IHC, Western blot
Immunohistochemistry of paraffin-embedded human heart tissue using antibody at 1:100 dilution.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human stomach tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Immunohistochemical analysis of SCOT staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

OXCT1 antibody LS-C81248 is an unconjugated rabbit polyclonal antibody to OXCT1 from human, mouse, rat and other species. Validated for WB.
About OXCT1
The mitochondrial matrix enzyme 3-oxoacid CoA transferase is homodimeric. It is a key enzyme in the extrahepatic utilization of ketone bodies, catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate, a necessary step in ketolytic energy production. Deficiencies can result in intermittent ketoacidosis. P55809 NM_000436 NP_000427.1

OXCT1 Antibody, 3-oxoacid CoA transferase 1 Antibody, 3-oxoacid CoA-transferase 1 Antibody, 3-oxoacid-CoA transferase 1 Antibody, 3-oxoacid CoA transferase Antibody, OXCT Antibody, SCOT Antibody, SCOT-s Antibody


Specifications

Target
Human OXCT1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Dog, Horse, Pig, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
OXCT1 antibody was raised against synthetic peptide located between aa316-365 of human OXCT1 (P55809, NP_000427). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Opossum, Guinea pig, Chicken, Platypus, Xenopus (100%); Panda, Dog, Cat, Horse, Pig, Stickleback, Zebrafish, Sea squirt (92%); Drosophila, Beetle (84%).
Blocking Peptide
LS-E17539 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81248. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa316-365
Specificity
Human OXCT1
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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