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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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OTUB1 / OTU1 Antibody (Biotin) LS‑C211851
OTU1 antibody LS-C211851 is a biotin-conjugated rabbit polyclonal antibody to human OTU1 (OTUB1). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C211851-50
50 µg
Unavailable
LS-C211851-100
100 µg
Unavailable

Popular OTUB1 / OTU1 Products

Antibody (0.05 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Human pancreas tissue using anti-OTUB1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OTUB1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded Human bladder tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Pig
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

OTU1 antibody LS-C211851 is a biotin-conjugated rabbit polyclonal antibody to human OTU1 (OTUB1). Validated for ELISA, IHC and WB.
About OTUB1 / OTU1
Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. Q96FW1 NM_017670 NP_060140.2

OTUB1 Antibody, Deubiquitinating enzyme OTUB1 Antibody, HSPC263 Antibody, HOTU1 Antibody, OTU-domain Ubal-binding 1 Antibody, OTU1 Antibody, Otubain-1 Antibody, Ubiquitin thioesterase OTUB1 Antibody, OTB1 Antibody


Specifications

Target
Human OTUB1 / OTU1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with HRP, FITC.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
OTUB1 / OTU1 antibody was raised against recombinant human Ubiquitin thioesterase OTUB1 protein.
Specificity
Human OTUB1 / OTU1
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Ubiquitin thioesterase OTUB1 antibody at 2 ug/ml. Lane 1: EC109 whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 20 kDa. Observed band size: 80 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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